Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: Succinate potentiated the production of CCL1 in CD4 + T cells to promote myofibroblast activation. ( A ) Heatmap showing the expression of Pde1a, Ccl1, Ccl3 , and Klb in control and succinate-treated CD4 + T cells. ( B ) qPCR analysis for Ccl1 and Ccl3 in CD4 + T cells treated with DMSO or dimethyl succinate (DS) (n = 6). ( C ) Immunoblot and ( D ) quantification analysis of CCL1 in the UUO kidneys from mice treated with succinate or DMM (n = 3). ( E ) Immunoblot and ( F ) quantification analysis of Col I and αSMA in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( G ) Immunoblot and ( H ) quantification analysis of pERK and ERK in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( I ) qPCR analysis for Col1a2 and Acta2 in NIH-3T3 fibroblasts co-cultured with succinate-preconditioned CD4 + T cells or untreated CD4 + T cells (n = 5). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Fibroblast activation was induced using recombinant human TGF-β1 (MCE, HY-P7118, 10ng/mL) and recombinant mouse CCL1 (MCE, HY- P73913 , 10ng/mL).

Techniques: Activation Assay, Expressing, Control, Western Blot, Cell Culture

Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: CCL1 promoted UUO-induced renal fibrosis. ( A ) The schematic of the experimental design. ( B ) Immunoblot and ( C ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and rmCCL1-treated mice (n = 3). ( D ) Masson staining and quantification of UUO kidneys from control and rmCCL1-treated mice (n = 5), scale bar = 40 μm. ( E ) qPCR analysis for Kim1 in the UUO kidneys from control and rmCCL1-treated mice (n = 5). ( F ) Immunoblot and ( G ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and anti-CCL1-treated mice (n = 3). ( H ) Immunoblot and ( I ) quantification analysis of Col I and αSMA expression in UUO kidneys from mice transferred with untreated or succinate-treated CD4 + T cells (n = 3). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Fibroblast activation was induced using recombinant human TGF-β1 (MCE, HY-P7118, 10ng/mL) and recombinant mouse CCL1 (MCE, HY- P73913 , 10ng/mL).

Techniques: Western Blot, Expressing, Control, Staining

Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: Succinate potentiated the production of CCL1 in CD4 + T cells to promote myofibroblast activation. ( A ) Heatmap showing the expression of Pde1a, Ccl1, Ccl3 , and Klb in control and succinate-treated CD4 + T cells. ( B ) qPCR analysis for Ccl1 and Ccl3 in CD4 + T cells treated with DMSO or dimethyl succinate (DS) (n = 6). ( C ) Immunoblot and ( D ) quantification analysis of CCL1 in the UUO kidneys from mice treated with succinate or DMM (n = 3). ( E ) Immunoblot and ( F ) quantification analysis of Col I and αSMA in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( G ) Immunoblot and ( H ) quantification analysis of pERK and ERK in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( I ) qPCR analysis for Col1a2 and Acta2 in NIH-3T3 fibroblasts co-cultured with succinate-preconditioned CD4 + T cells or untreated CD4 + T cells (n = 5). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: To investigate the in vivo functional role of CCL1, we administered recombinant murine CCL1 (MCE, HY- P73913 , 1 mg/kg every 3 days) and a CCL1-neutralizing antibody (Invitrogen, PA5-47952, 50 μg/kg every 3 days) into UUO mice.

Techniques: Activation Assay, Expressing, Control, Western Blot, Cell Culture

Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: CCL1 promoted UUO-induced renal fibrosis. ( A ) The schematic of the experimental design. ( B ) Immunoblot and ( C ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and rmCCL1-treated mice (n = 3). ( D ) Masson staining and quantification of UUO kidneys from control and rmCCL1-treated mice (n = 5), scale bar = 40 μm. ( E ) qPCR analysis for Kim1 in the UUO kidneys from control and rmCCL1-treated mice (n = 5). ( F ) Immunoblot and ( G ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and anti-CCL1-treated mice (n = 3). ( H ) Immunoblot and ( I ) quantification analysis of Col I and αSMA expression in UUO kidneys from mice transferred with untreated or succinate-treated CD4 + T cells (n = 3). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: To investigate the in vivo functional role of CCL1, we administered recombinant murine CCL1 (MCE, HY- P73913 , 1 mg/kg every 3 days) and a CCL1-neutralizing antibody (Invitrogen, PA5-47952, 50 μg/kg every 3 days) into UUO mice.

Techniques: Western Blot, Expressing, Control, Staining

Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: Succinate potentiated the production of CCL1 in CD4 + T cells to promote myofibroblast activation. ( A ) Heatmap showing the expression of Pde1a, Ccl1, Ccl3 , and Klb in control and succinate-treated CD4 + T cells. ( B ) qPCR analysis for Ccl1 and Ccl3 in CD4 + T cells treated with DMSO or dimethyl succinate (DS) (n = 6). ( C ) Immunoblot and ( D ) quantification analysis of CCL1 in the UUO kidneys from mice treated with succinate or DMM (n = 3). ( E ) Immunoblot and ( F ) quantification analysis of Col I and αSMA in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( G ) Immunoblot and ( H ) quantification analysis of pERK and ERK in NIH-3T3 cells treated with PBS or rmCCL1 (n =3). ( I ) qPCR analysis for Col1a2 and Acta2 in NIH-3T3 fibroblasts co-cultured with succinate-preconditioned CD4 + T cells or untreated CD4 + T cells (n = 5). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: To investigate the in vivo functional role of CCL1, we administered recombinant murine CCL1 (MCE, HY- P73913 , 1 mg/kg every 3 days) and a CCL1-neutralizing antibody (Invitrogen, PA5-47952, 50 μg/kg every 3 days) into UUO mice.

Techniques: Activation Assay, Expressing, Control, Western Blot, Cell Culture

Journal: Journal of Inflammation Research

Article Title: Succinate Facilitates CD4 + T Cell Infiltration and CCL1 Production to Promote Myofibroblast Activation and Renal Fibrosis in UUO Mice

doi: 10.2147/JIR.S510637

Figure Lengend Snippet: CCL1 promoted UUO-induced renal fibrosis. ( A ) The schematic of the experimental design. ( B ) Immunoblot and ( C ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and rmCCL1-treated mice (n = 3). ( D ) Masson staining and quantification of UUO kidneys from control and rmCCL1-treated mice (n = 5), scale bar = 40 μm. ( E ) qPCR analysis for Kim1 in the UUO kidneys from control and rmCCL1-treated mice (n = 5). ( F ) Immunoblot and ( G ) quantification analysis of Col I and αSMA expression in UUO kidneys from control and anti-CCL1-treated mice (n = 3). ( H ) Immunoblot and ( I ) quantification analysis of Col I and αSMA expression in UUO kidneys from mice transferred with untreated or succinate-treated CD4 + T cells (n = 3). The results represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: To investigate the in vivo functional role of CCL1, we administered recombinant murine CCL1 (MCE, HY- P73913 , 1 mg/kg every 3 days) and a CCL1-neutralizing antibody (Invitrogen, PA5-47952, 50 μg/kg every 3 days) into UUO mice.

Techniques: Western Blot, Expressing, Control, Staining